Microinjection of chemical carcinogens into small fish embryos: exocrine pancreatic neoplasm
نویسنده
چکیده
Embryos of gulf killifish Fundulus grandis were injected with N-methyl-N'-nitro-Nnitrosoguanidine (MNNG). Retention of carcinogen in the egg immediately after injection was highly variable. Hatching percentages of MNNG-injected and uninjected embryos were similar, but survival of MNNG-injected fish during the first week after hatching was less than half of the survival of controls. One of 3 MNNG-exposed fish examined after 20 wk had a pancreatic acinar cell carcinoma, a type of neoplasm seldom found in fish. No neoplasms were recorded in controls nor in MNNG-exposed fish examined after 34 wk. The neoplasm was invasive and had a high mitotic index, but no metastases were found. The acinar cell origin of the tumor was confirmed by ultrastructural examination of tissue reprocessed from paraffin into epoxy resin.
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